mouse anti atoh1  (Developmental Studies Hybridoma Bank)

Journal: Life

Article Title: Neurogenic Stem Cell Niche in the Auditory Thalamus: In Vitro Evidence of Neural Stem Cells in the Rat Medial Geniculate Body

doi: 10.3390/life13051188

Figure Lengend Snippet: The expression of neural stem cell markers in neurospheres from cells of the PND 8 MGB. ( a – d ) Cells inside the neurospheres and cells emigrating from the spheres were stained positively for the neural progenitor cell marker nestin (red); cell nuclei were stained blue with DAPI. ( a ) The nuclei of cells inside the sphere and its branches showed positive labeling for the transcription factor ATOH1 (green). ( b ) The cytoplasm of the out-migrating cells and those inside the neurospheres were stained with the neural migration protein DCX (green). ( c ) The neural stem cell marker Musashi-1 (green) was positive in the cytoplasm of cells within the neurospheres and their branches. ( d ) The nuclei in the neurospheres and their branches showed positive labeling for the transcription factor Sox-2 (green).

Article Snippet: Incubation with the following primary antibodies were performed for immunocytochemical assays at 5 °C for 12 h in 1% BSA solution and 0.1 M PBS buffer: mouse monoclonal antibody against ATOH1 (1:1000; Ab27667-Abcam ® , Waltham, MA, USA), mouse monoclonal against β-tubulin (1:1000; #TS293—Sigma-Aldrich ® , Merck ® , St. Louis, MO, USA), mouse monoclonal versus β-III-tubulin (1:1000; #Ab7751—Abcam ® , Waltham, MA, USA), rabbit polyclonal versus β-III-tubulin (1:2000; #Ab18207—Abcam ® , Waltham, MA, USA), rabbit polyclonal against double cortin (DCX) (1:1000; #Ab18723—Abcam ® , Waltham, MA, USA), mouse monoclonal protein against glial fibrillary acidic protein (GFAP) (1:1000; #MAB360—Merck Millipore ® , Billerica, MA, USA), rabbit polyclonal against myelin basic protein (MBP) (1:800; #M3821—Sigma-Aldrich ® , Merck ® , St. Louis, MO, USA), rabbit polyclonal versus Musashi-1 (1:100, Abcam ® , Waltham, MA, USA), mouse monoclonal protein versus nestin (1:800; #MAB353—Merck Millipore ® , Billerica, MA, USA), and rabbit polyclonal versus Sox-2 (1:2000; #Ab97959—Abcam ® , Waltham, MA, USA).

Techniques: Expressing, Staining, Marker, Labeling, Migration

Journal: Life

Article Title: Neurogenic Stem Cell Niche in the Auditory Thalamus: In Vitro Evidence of Neural Stem Cells in the Rat Medial Geniculate Body

doi: 10.3390/life13051188

Figure Lengend Snippet: Neural stem cell markers are detectable in in vivo tissue sections of the MGB. ( a ) The transcription factor ATOH1 (red) is expressed in the nucleus of cells, and the cytoplasm of cells was positive for ß-tubulin (green). Cell nuclei are stained blue by DAPI. ( c ) The transcription factor Sox-2 (red) is expressed in the nucleus of cells. β-tubulin (red) is expressed in the cytoplasm of the cells. Cell nuclei are stained blue by DAPI. ( b ) Results of the immunohistochemical analysis of the neural stem cell markers ATOH1 and Sox-2 in histological sections of the PND 8 rat MBG. The proportions of positively stained cells in relation to the total number of cells on coverslips were evaluated. The bar charts show the mean and Standard Error of the Mean (SEM).

Article Snippet: Incubation with the following primary antibodies were performed for immunocytochemical assays at 5 °C for 12 h in 1% BSA solution and 0.1 M PBS buffer: mouse monoclonal antibody against ATOH1 (1:1000; Ab27667-Abcam ® , Waltham, MA, USA), mouse monoclonal against β-tubulin (1:1000; #TS293—Sigma-Aldrich ® , Merck ® , St. Louis, MO, USA), mouse monoclonal versus β-III-tubulin (1:1000; #Ab7751—Abcam ® , Waltham, MA, USA), rabbit polyclonal versus β-III-tubulin (1:2000; #Ab18207—Abcam ® , Waltham, MA, USA), rabbit polyclonal against double cortin (DCX) (1:1000; #Ab18723—Abcam ® , Waltham, MA, USA), mouse monoclonal protein against glial fibrillary acidic protein (GFAP) (1:1000; #MAB360—Merck Millipore ® , Billerica, MA, USA), rabbit polyclonal against myelin basic protein (MBP) (1:800; #M3821—Sigma-Aldrich ® , Merck ® , St. Louis, MO, USA), rabbit polyclonal versus Musashi-1 (1:100, Abcam ® , Waltham, MA, USA), mouse monoclonal protein versus nestin (1:800; #MAB353—Merck Millipore ® , Billerica, MA, USA), and rabbit polyclonal versus Sox-2 (1:2000; #Ab97959—Abcam ® , Waltham, MA, USA).

Techniques: In Vivo, Staining, Immunohistochemical staining